
All lanes : Anti-SOX2 antibody - Embryonic Stem Cell Marker ( ab15830 ) at 1 µg/ml Lane 1 : 46C (Mouse neural progenitor, selected for Sox1 expression cell line) Whole Cell Lysate (ab27205) Lane 2 : IOUD2 (Mouse embryonic stem cell, selected for Oct4 expression cell line) Whole Cell Lysate ( ab27202 ) Lane 3 : F9 (Mouse embryonic carcinoma cell line) Whole Cell Lysate ( ab27193 ) Lane 4 : 46C (Mouse neural progenitor, selected for Sox1 expression cell line) Whole Cell Lysate (ab27205) with Human SOX2 peptide ( ab15831 ) at 1 µg/ml Lane 5 : IOUD2 (Mouse embryonic stem cell, selected for Oct4 expression cell line) Whole Cell Lysate ( ab27202 ) with Human SOX2 peptide ( ab15831 ) at 1 µg/ml Lane 6 : F9 (Mouse embryonic carcinoma cell line) Whole Cell Lysate ( ab27193 ) with Human SOX2 peptide ( ab15831 ) at 1 µg/ml Lysates/proteins at 10 µl per lane. Observed band size : 42 kDa ( why is the actual band size different from the predicted? )